Kwaikwayi yanayin ilimin lissafi yana taimaka wa masu bincike su sami masu ɗaure ƙarfe
Masu bincike sun kirkiro wata hanya ta gano kananan kwayoyin da ke daure ion karfe.Ƙarfe ions suna da mahimmanci a ilmin halitta.Amma gano waɗanne ƙwayoyin cuta-kuma musamman waɗanne ƙananan ƙwayoyin cuta-wadanda ions na ƙarfe ke hulɗa da su na iya zama ƙalubale.
Don raba metabolites don bincike, hanyoyin metabolomics na al'ada suna amfani da kaushi na halitta da ƙananan pHs, wanda zai iya haifar da hadaddun ƙarfe don rarrabuwa.Pieter C. Dorrestein na Jami'ar California San Diego da abokan aikinsa sun so su ci gaba da hada hadaddun tare don bincike ta hanyar kwaikwayon yanayin asalin da ake samu a cikin sel.Amma da a ce sun yi amfani da yanayin physiological lokacin rabuwar kwayoyin halitta, da sun sake inganta yanayin rabuwa na kowane yanayin da suke son gwadawa.
Madadin haka, masu binciken sun haɓaka matakan matakai guda biyu waɗanda ke gabatar da yanayin ilimin lissafin jiki tsakanin rarrabuwa na chromatographic na al'ada da ƙididdigar ƙima (Nat. Chem. 2021, DOI: 10.1038 / s41557-021-00803-1).Na farko, sun raba wani tsantsa nazarin halittu ta amfani da al'ada high-performance ruwa chromatography.Daga nan sai suka daidaita pH na kwararar da ke fitowa daga ginshiƙi na chromatographic don kwaikwayi yanayin ilimin lissafi, ƙara ions na ƙarfe, kuma sun yi nazarin cakuda tare da ma'auni mai yawa.Sun gudanar da bincike sau biyu don samun yawan adadin ƙananan ƙwayoyin cuta tare da ba tare da karafa ba.Don gano waɗanne ƙwayoyin ƙwayoyin cuta ne ke ɗaure karafa, sun yi amfani da hanyar ƙididdigewa wacce ke amfani da sifofi kololuwa don ba da alaƙa tsakanin sifofin da aka ɗaure da waɗanda ba a ɗaure ba.
Hanya ɗaya don ƙara kwaikwaya yanayin ilimin lissafi, Dorrestein ya ce, zai kasance ƙara yawan ions kamar sodium ko potassium da ƙananan ƙarancin ƙarfe na ban sha'awa."Ya zama gwajin gasa.A zahiri zai gaya muku, Ok, wannan kwayar halitta a ƙarƙashin waɗannan yanayi yana da ƙarin kusanci don ɗaure sodium da potassium ko wannan ƙarfe na musamman da kuka ƙara,” in ji Dorrestein."Za mu iya shigar da karafa daban-daban a lokaci guda, kuma za mu iya fahimtar fifiko da zaɓi a cikin mahallin."
A cikin al'adun da aka samo daga Escherichia coli, masu binciken sun gano sanannun mahadi masu ɗaure baƙin ƙarfe irin su yersiniabactin da aerobactin.Game da yersiniabactin, sun gano cewa yana iya ɗaure zinc.
Masu binciken sun gano mahadi masu ɗaure ƙarfe a cikin samfuran a matsayin masu rikitarwa kamar narkar da kwayoyin halitta daga cikin teku."Wannan shine gaba ɗaya ɗaya daga cikin mafi hadaddun samfuran da na taɓa kallo," in ji Dorrestein."Wataƙila yana da sarƙaƙiya kamar, idan bai fi ɗanyen mai rikitarwa ba."Hanyar ta gano domoic acid a matsayin kwayar tagulla mai ɗaure tagulla kuma ta ba da shawarar cewa ya ɗaure Cu2+ azaman dimer.
Oliver Baars, wanda ke nazarin abubuwan da ke tattare da karfe da tsire-tsire da microbes ke samarwa a Jami'ar Jihar North Carolina ya rubuta cewa: "Tsarin omics don gano duk metabolites masu ɗaure ƙarfe a cikin samfurin yana da matukar amfani saboda mahimmancin chelation na halitta. imel.
"Dorrestein da abokan aiki suna ba da kyakkyawan tsari, da ake buƙata sosai, don bincika mafi kyawun abin da aikin physiological na ions na ƙarfe a cikin tantanin halitta zai iya zama," Albert JR Heck, majagaba a cikin nazarin ƙididdigar yawan jama'a a Jami'ar Utrecht, ya rubuta a cikin imel."Mataki na gaba mai yuwuwa shine a fitar da metabolites a ƙarƙashin yanayin asali daga tantanin halitta kuma a raba su a ƙarƙashin yanayin ƙasa, don ganin waɗanne metabolites ke ɗauke da ions ƙarfe na salula."
Labaran Kimiyya & Injiniya
Saukewa: ISSN009-2347
Haƙƙin mallaka © 2021 American Chemical Society
Lokacin aikawa: Dec-23-2021